Research:

I formally joined the Herzenberg Laboratory in April 2002. My work is focused on the development of Hi-D FACS assays for human lung diseases, with a particular emphasis on cystic fibrosis (CF), which I have worked on for several years (see Publications). I am currently exploring the association between cellular redox imbalances and CF physiopathology in vivo and beyond, how the redox balance regulates lung immunity in humans. This work involves a close collaboration with the CF Clinic at Stanford (head: Dr Richard Moss). I also recently started a project aimed at better understanding Drosophila immunity using Hi-D FACS, in collaboration with Dr Ranjiv Khush (Schneider Lab, Stanford).

Quick history: From 1999 to 2002, I have been working in the Cystic Fibrosis Research Lab at Stanford University (head: Dr Jeffrey J. Wine). My project was centered on the validation of a new model of human small airway epithelium and on the relation between glutathione and CF lung disease. From 1994 to 1999, I did my PhD studies within the Institute of Embryology of CNRS and College de France near Paris (head: Pr Nicole Le Douarin) under the direction of Dr Bruno Peault. My PhD work consisted in the set-up of a xenograft model of human airways using the Severe Combined Immunodeficient (SCID) mouse as a host.

Publications:

Tirouvanziam R., Hodson A., Bastacky J., Herzenberg L. and Wine J. (2002). An air interface model of human small airways shows positive correlation between CFTR and ENaC activities. (in preparation)

Tirouvanziam R., Khazaal I. and Péault B. (2002). Primary inflammation in human cystic fibrosis small airways. Am. J. Physiol. (Lung Cell Mol. Physiol.): in press [DOI,10.1152 /ajplung.00419.2001].

Tirouvanziam R., Khazaal I., N'Sondé V., Peyrat M.-A., Lim A., de Bentzmann S., Fournié J.-J., Bonneville M. and Péault B. (2002). Ex vivo development of functional human lymph node and bronchus-associated lymphoid tissue. Blood 99 (7): 2483-89.

Tirouvanziam R., De Bentzmann S., Hinnrasky J., Jacquot J., Péault B. and Puchelle E. (2000). Inflammation and infection in naïve human cystic fibrosis airway grafts. Am. J. Respir. Cell Mol. Biol. 23 (2): 121-127.

Delplanque A., Coraux C., Tirouvanziam R., Khazaal I., Puchelle E., Ambros P., Gaillard D. and Péault B. (2000). Epithelial stem cell-mediated development of the human respiratory mucosa in SCID mice. J. Cell Sci. 113 (Pt 5): 767-778.

Tirouvanziam R. (1999). Cystic fibrosis: a complex disease and a paradigm for biomedical research. J. Soc. Biol. 193 (2):189-209 [Review (French)]

Baconnais S., Tirouvanziam R., Zahm J.-M., de Bentzmann S., Péault B., Balossier G. and Puchelle E. (1999). Ion composition and rheology of airway liquid from cystic fibrosis fetal tracheal xenografts. Am. J. Respir Cell. Mol. Biol. 20 (4):605-11.

Tirouvanziam R., Desternes M., Saari A., Puchelle E., Péault B. and Chinet T. (1998). Bioelectric properties of human cystic fibrosis and non-cystic fibrosis fetal tracheal xenografts in SCID mice. Am. J. Physiol. (Cell. Physiol.) 274(4 Pt 1):C875-82.

Tirouvanziam R. and Péault B. (1995). Gene transfer to human respiratory airways developed in SCID mice. Biologicals. 23(1):17-9. Review.

Peault B., Tirouvanziam R., Sombardier M.-N., Chen S., Perricaudet M. and Gaillard D. (1994). Gene transfer to human fetal pulmonary tissue developed in immunodeficient SCID mice. Hum. Gene Ther. 5(9):1131-7.