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Mitchell, H. K. and Herzenberg, L. A. (1955). Enzymatic degradation of cytohrome C. Methods of Enzymology. C. a. Kaplan. New York, Academic Press. 60: 167-169.
Mitchell, H. K. and Herzenberg, L. A. (1957). “Zone electrophoresis on sponge rubber.” Anal. Chem 29(8): 1229.
Herzenberg, L. A., Herzenberg, L.A. (1959). “Adaption to Lactose.” Nutrition Reviews 17: 65-67.
Herzenberg, L. A. (1959). “Studies on the induction of b-galactosidase in a cryptic strain of escherichia coli.” Biochem. et Biophys. Acta 31: 525-538.
Herzenberg, L. A. (1960). Discussion of genetics of somatic cells. Methodology in Mammalian Genetics. San Francisco, Holden-Day, Inc.: 462-463.
Herzenberg, L. A., R.A. Roosa (1959). “Nutritional Requirements for growth of a mouse lymphona in cell culture.” Exp. Cell Res. 21: 430-438.
Herzenberg, L. A. (1961). Isolation and Identificatio of Derivatives Formed in the Course of Intracellular Accumulation of Thiogalactosides by Escherichia coli. Archives of Biochemistry and Biophysics. 93: 314-315.
LAH #008
Cann, H. M., Herzenberg, L.A. (1961). “Detection and Localization of H-2 Antigen in Cells Grown in Culture.” Am. J. Diseases of Children 102(4): 477.
Herzenberg, L. A. (1962). Chemical and Serological Characterization of Purified H-2 Antigens. Mechanisms of Immunological Tolerance. Prague, Publishing House of the Czechoslovak Academy of Sciences: 495-499.
Ames, B., Garry, B. and Herzenberg, L. (1961). “Association of H-2 antigens with the cell membrane fraction of mouse liver.” Proc. Nat. Acad. Sci 47: 762-767.
LAH #010
Herzenberg, L. A., Herzenberg, L.A. (1961). “Association of H-2 Antigens with the Cell Membrane Fraction of Mouse Liver.” Proceedings of the National Academy of Sciences 47: 762-767.
Herzenberg, L. A., Gonzales, B. (1962). “Appearance of H-2 Agglutinins in Outcrossed Female Mice.” Proceedings of the National Academy of Sciences 48(4): 570-573.
LAH #012
Herzenberg, L. A. (1962). Histocompatibility Antigens Tissue Transplantation. American Conference of Physicians, Annual Meeting.
Herzenberg, L. A. (1962). A Genetic and Immunologic Approach to the Purification of an Histocompatibility Antigen. The Effects of Ionizing Radiations on Immune Processes. C. A. Leone. New York, Gordon and Breach Science Publishers: 365-379.
Ozer HL, H. L. (1962). “H-2 Immunogenicity of Liver Cell Membranes in the Mouse.” Transplantation Bulletin 30(1): 165/52-54.
LAH #015
Cann, H. M., Herzenberg, L.A. (1962). “In Vitro Selection for Isoantigenic Variants of Mammalian Somatic Cells.” Am. J. Diseases of Children: 533.
LAH #015-01
LAH #016
Herzenberg, L. A. (1962). “Steps Toward a Genetics of Somatic Cells in Culture.” J. of Cellular and Comparative Physiology 60(2): 145-151.
LAH #017
Herzenberg, L. A. (1962). “Maternal Isoimmunization as a Result of breeding in the Mouse.” J. of Cellular and Comparative Psyiology 60(2): 152-157.
Roosa, R. A., Bradley, T.R., Law, L.W., Herzenberg, L.A. (1962). “Characterization of Resistance to Amethopterin 8-Azaguanine and Several Fluorinated Pyrimidines in the Murine Lymphocytic Neoplasm, P388.” J. of Cellular and Comparative Physiology 60(2): 109-126.
LAH #019
Wunderlich, J. R., Herzenberg, L.A. (1962). “A second Gamma Globulin Isoatigen (allotype) in the Mouse.” Genetics 47: 995.
Cann, H. M., Herzenberg, L.A. (1963). “In Vitro Studies of Mammalian Somatic Cells.” The J. of Exp. Med. 117(2): 259-265.
LAH #020-01
Goodlin, R. and Herzenberg, L. (1964). “Pregnancy induced hemagglutinins to paternal H-2 antigens in the multiparous mice.” Transplantiation 2: 357-361.
LAH #020-02
Goodlin, R., Herzenberg, L. and De'Ath, S. (1964). “Isoimmunization associtaed with Ceserian section in the mouse.” Am. J. Obst. and Gynec. 90: 776-778.
Cann, H. M., Herzenberg, L.A. (1963). “In Vitro Studies of Mammalian Somatic Cell Variations. II” The J. of Exp. Med. 117(2): 267-284.
Mishell, R. I., Herzenberg, L.A., Herzenberg, L.A. (1962). “Leukocyte Agglutination in Mice: Detection of H-2 and non-H-2 Isoantigens.” The J. of Immun. 90(4): 628-633.
Herzenberg, L. A., Tachibana, D.K., Herzenberg, L.A. (1963). “A Gene Locus Concerned with Hemolytic Complement in Mus Musculus.” Genetics 48(5): 711-715.
Wunderlich, J., Herzenberg, L.A. (1963). “Genetics of a Gamma Globulin Isoantigen (Allotype) in the Mouse.” Proceedings of the National Academy of Sciences. 49(5): 592-598.
LAH #025
Herzenberg, L. A., Rosenberg, P., Herzenberg, L.A. (1963). “Gamma globulin isoantigens (allotypes) in the mouse.” Genetics 48: 892.
LAH #026
Herzenberg, L. A., Mishell, R.I., Herzenberg, L.A. (1963). “Gamma-globulin Isoantigens (Allotypes) in the House Mouse.” Proc. of the XI Intern. Congr. of Genetics, The Hague I: 196.
LAH #027
Warner, N. L., Herzenberg, L. A. and Goldstein, G. (1966). “Immunoglobulin isoantigens (allotypes) in the mouse. I. Genetics and Cross-Reaction of the 7S g2A-Isoatigens Controlled by Alleles at the Ig-1 Locus.” Journal of Experimental Medicine 121(3): 415-438.
Erickson, R. P., Herzenberg, L.A., Goor, R. (1964). “Partial Immune Elimination of Homologous Red Blood Cells in Mice.” Transplantation 2(2): 175-182.
Herzenberg, L. A., Cole, L.J. (1964). “Presence of Donor Specific Gamma-globulins in Sera of Allogeneic Mouse Radiation Chimeras.” Nature 202(4930): 352-353.
LAH #028-03
Goodlin, R. C. and Herzenberg, L. (1966). “Further studies on the mechanism of pregnancy-induced isoimmunization in mice.” American Journal of Obstetrics & Gynecology 95(1): 133-4.
Erickson, R. P., Tavhibana, D.K., Herzenberg, L.A., Rosenberg, L.T. (1964). “A Single Gene Controlling Hemolytic Compliment and a Serum Antigen in the Mouse.” J. of Immun. 92(4): 611-615.
Papermaster, B. W. and Herzenberg, L. A. (1966). “Isolation and characterization of an isoantigenic variant from a heterozygous mouse lymphoma in culture.” Journal of Cellular Physiology 67(3): 407-20.
Herzenberg, L. A. (1965). Antigen Variations of Samatic Cells and Cytogenic Aspects of Cell Antigenicity - Introduction. Genetic Variations in Somatic Cells - Proceedings of the Symposium on the mutational Process. Praha, Academia: 363-372.
Herzenberg, L. A., Herzenberg, L.A., (1965). Suppression of a gG-globulin allotype in mice by anti-allotype antibodies. Genetic Variations in Somatic Cells. Praha, Academia: 227-232.
Warner, N. L., Herzenberg, L. A. and Goldstein, G. (1966). “Immunoglobulin isoantigens (allotypes) in the mouse. II. Allotypic analysis of three gammaG2-myeloma proteins from (NZB x BALB/c)F1 hybrids and of normal gammaG2-globulins.” Journal of Experimental Medicine 123(4): 707-21.
LAH #033
Warner, N. L. and Herzenberg, L. A. (1966). “Immunoglobulin isoantigens (allotypes) in the mouse. 3. Detection of allotypic antigens with heterologous antisera.” Journal of Immunology 97(4): 525-31.
Warner, N. L., Herzenberg, L. A., Cole, L. J. and Davis, W. E., Jr. (1965). “Dissociation of skin homograft tolerance and donor type gamma globulin synthesis in allogeneic mouse radiation chimaeras.” Nature 205(976): 1077-9.
Tyan, M. L., Cole, L. J. and Herzenberg, L. A. (1967). “Fetal liver: a source of immunoglobulin producing cells in the mouse.” Proceedings of the Society for Experimental Biology & Medicine 124(4): 1161-3.
Klein, J., Martinkova, J. and Herzenberg, L. A. (1967). “Analysis of the histocompatibility-2 (H-2) locus of NZB mice.” Transplantation 5(5): 1335-8.
Klein, J. and Herzenberg, L. A. (1967). “Congenic mouse strains with different immunoglobulin allotypes. I. Breeding scheme, histocompatibility tests, and kinetics of gamma G2a-globulin production by transferred cells for C3H.SW and its congenic partner CWB/5.” Transplantation 5(6): 1484-95.
Welton, J., Walker, S. R., Sharp, G. C., Herzenberg, L. A., Wistar, R., Jr. and Creger, W. P. (1968). “Macroglobulinemia with bone destruction.” American Journal of Medicine 44(2): 280-8.
Warner, N. L. and Herzenberg, L. A. (1967). “Immunoglobulin isoantigens (allotypes) in the mouse. IV. Allotypic specificities common to two distinct immunoglobulin classes.” Journal of Immunology 99(4): 675-8.
Minna, J. D., Iverson, G. M. and Herzenberg, L. A. (1967). “Identification of a gene locus for gamma-G-1 immunoglobulin H chains and its linkage to the H chain chromosome region in the mouse.” Proceedings of the National Academy of Sciences of the United States of America 58(1): 188-94.
Tyan, M. L., Cole, L. J. and Herzenberg, L. A. (1967). “Fetal liver cells: a source of specific immunoglobulin production in radiation chimeras. USNRDL-TR-67-26.” Research & Development Technical Report: 1-8.
Herzenberg, L. A., Goodlin, R. C. and Rivera, E. C. (1967). “Immunoglobulin synthesis in mice: suppression by anti-allotype antibody.” Journal of Experimental Medicine 126(4): 701-13.
LAH #043
Herzenberg, L. A., Minna, J.D., Herzenberg, L.A. (1967). “The Chromosome Region for Immunoglobulin Heavy Chains in the Mouse: Allelic Electrophoretic Mobility Differences and Allotype Suppression.” Cold SPring Harbor Symposia on Quantitative Biology XXXII: 181-186.
Herzenberg, L. A., H.O. McDevitt, L.A. Herzenberg (1968). “Genetics of Antibodies.” Annual Review of Genetics 2: 209-244.
Tyan, M. L. and Herzenberg, L. A. (1968). “Ontogeny of the mouse immune system: immunoglobulin producing cells. USNRDL-TR-68-57.” Research & Development Technical Report: 1-14.
LAH #045-01
Tyan, M. L. and Herzenberg, L. A. (1968). “Studies on the ontogeny of the mouse immune system. II. Immunoglobulin-producing cells.” Journal of Immunology 101(3): 446-50.
Tyan, M. L., McDevitt, H. O. and Herzenberg, L. A. (1969). “Genetic control of the antibody response to a synthetic polypeptide: transfer of response with spleen cells or lymphoid precursors.” Transplantation Proceedings 1(1): 548-50.
Tyan, M., Herzenberg, L. and Cole), I. b. L. J. (1968). Immunoglobulin Production by Embryonic Tissues: Thymus Independent. Proceedings of the Society for Experimental Biology and Medicine. 12: 952-954.
Hulett, H. R., Bonner, W. A., Barrett, J. and Herzenberg, L. A. (1969). “Cell sorting: automated separation of mammalian cells as a function of intracellular fluorescence.” Science 166(906): 747-9.
Tyan, M. L., Herzenberg, L. A. and Gibbs, P. R. (1969). “Lymphoid precursors: thymus independent antibody production.” Journal of Immunology 103(6): 1283-7.
L'Age-Stehr, J. and Herzenberg, L. A. (1970). “Immunological memory in mice. I. Physical separation and partial characterization of memory cells for different immunoglobulin classes from each other and from antibody-producing cells.” Journal of Experimental Medicine 131(6): 1093-108.
Jacobson, E. B., L'Age-Stehr, J. and Herzenberg, L. A. (1970). “Immunological memory in mice. II. Cell interactions in the secondary immune response studies by means of immunoglobulin allotype markers.” Journal of Experimental Medicine 131(6): 1109-20.
Riblet, R. J. and Herzenberg, L. A. (1970). “Mouse lysozyme production by a monocytoma: isolation and comparison with other lysozymes.” Science 168(939): 1595-7.
Herzenberg, L. A. (1971). Gene Interaction in Immunglobulins. Human Anti-Human Gammaglobulins. G. a. Samuelsson. Oxford and New York, Pergamon Press: 3-15.
Chesebro, B. W., Mitchell, G. F., Grumet, F. C., Herzenberg, L. A. and McDevitt, H. O. (1970). Analysis of Cell Transfer Studies in a Genetic Control of the Immune Response in Mice. Cellular Interactions in the Immune Response. B. Karger. Buffalo, NY, Convoe Immunology: 83-92.
Herzenberg, L. A. (1970). “From cell biology to immunology--a short trip.” Journal of Cellular Physiology 76(3): 303-10.
Warner, N. L. and Herzenberg, L. A. (1970). “Tolerance and immunity to maternally derived incompatible IgG 2a -globulin in mice.” Journal of Experimental Medicine 132(3): 440-7.
Merrill, J. T., Veizades, N., Hulett, H. R., Wolf, P. L. and Herzenberg, L. A. (1971). “An improved cell volume analyzer.” Review of Scientific Instruments 42(8): 1157-63.
Mitchell, G. F., Chan, E. L., Noble, M. S., Weissman, I. L., Mishell, R. I. and Herzenberg, L. A. (1972). “Immunological memory in mice. 3. Memory to heterologous erythrocytes in both T cell and B cell populations and requirement for T cells in expression of B cell memory. Evidence using immunoglobulin allotype and mouse alloantigen theta markers with congenic mice.” Journal of Experimental Medicine 135(2): 165-84.
LAH #057-05
Chesebro, B. W., Mitchell, G. F., Grumet, F. C., Herzenberg, L. A., McDevitt, H. O. and Wegmann, T. G. (1972). “Cell transfer studies in a genetically controlled immune response.” European Journal of Immunology 2(3): 243-8.
Mitchell, G., Mishell, R. and Herzenberg, L. (1971). Studies on the influence of T cells in antibody production. Progress in Immunology. New York, Academic Press: 323-335.
Herzenberg, L. A., R.G. Mage (1971). Allotypes. II
Presented at the first International Congress of Immunology. Progress in Immunology. New York, Academic Press: 1249-1251.
Herzenberg, L. A. (1971). “Immunoglobulin genetics in cellular immunology.” Annals of the New York Academy of Sciences 190: 130-5.
Herzenberg, L. A., Jacobson, E. B. and Riblet, R. J. (1971). “Chronic allotype suppression in mice: an active regulatory process.” Annals of the New York Academy of Sciences 190: 212-20.
LAH #062
Herzenberg, L. A. (1971). Discussion of separation of cells. Immunologic Intervention: II selective depletion of lymphocytes. New York: 96-104,145-148,281,289.
Jacobson, E. B. and Herzenberg, L. A. (1972). “Active suppression of immunoglobulin allotype synthesis. I. Chronic suppression after perinatal exposure to maternal antibody to paternal allotype in (SJL x BALB-c)F 1 mice.” Journal of Experimental Medicine 135(5): 1151-62.
Bonner, W. A., Hulett, H. R., Sweet, R. G. and Herzenberg, L. A. (1972). “Fluorescence activated cell sorting.” Review of Scientific Instruments 43(3): 404-9.
Herzenberg, L. A., L.A. Herzenberg (1973). Mouse immunolglobulin allotypes: Description and special methodology. Handbook of Experimental Immunology 2nd Edition. Oxford, Blackwell Scientific Publications: 13.1-18.
Jacobson, E. B., Herzenberg, L. A. and Riblet, R. (1972). “Active suppression of immunoglobulin allotype synthesis. II. Transfer of suppressing factor with spleen cells.” Journal of Experimental Medicine 135(5): 1163-76.
Julius, M. H., Masuda, T. and Herzenberg, L. A. (1972). “Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter.” Proceedings of the National Academy of Sciences of the United States of America 69(7): 1934-8.
Leute, R. K., Ullman, E. F., Goldstein, A. and Herzenberg, L. A. (1972). “Spin immunoassay technique for determination of morphine.” Nature - New Biology 236(64): 93-4.
Freed, J. H., Bechtol, K. B., Herzenberg, L. A. and McDevitt, H. O. (1973). “Analysis of anti-(T,G)-A--L antibody in tetraparental mice.” Transplantation Proceedings 5(1): 167-71.
Sarkar, S., Hyman, R., Masuda, T. and Herzenberg, L. A. (1973). “A rabbit antiserum to a thymus extract specific for mouse thymus-derived cells.” Journal of Immunology 110(5): 1222-32.
Jones, P. P., Cebra, J. J. and Herzenberg, L. A. (1973). “Immunoglobulin (Ig) allotype markers on rabbit lymphocytes: separation of cells bearing different allotypes and demonstration of the binding of Ig to lymphoid cell membranes.” Journal of Immunology 111(5): 1334-48.
Jan, W. H. and Herzenberg, L. A. (1973). Fetal erythrocytes detected and separated from maternal blood by an electronic fluorescent cell sorter. SAMA-UTMB, National Student research Forum, Galveston, TX.
Hulett, H. R., Bonner, W. A., Sweet, R. G. and Herzenberg, L. A. (1973). “Development and application of a rapid cell sorter.” Clinical Chemistry 19(8): 813-6.
Bobrove, A. M., Strober, S., Herzenberg, L. A. and DePamphilis, J. D. (1974). “Identification and quantitation of thymus-derived lymphocytes in human peripheral blood.” Journal of Immunology 112(2): 520-7.
Herzenberg, L. A. (1973). “Curing Cancer by federal fiat.” Hospital Practice 8: 16.
Freed, J. H. and Herzenberg, L. A. (1975). “Quantitative separation of antigen-specific murine antibodies by anti-allotype chromatography.” Scandinavian Journal of Immunology 4(5-6): 479-85.
The use of Sepharose-conjugated murine anti-Iga or anti-Igb allo-antisera allowed the quantitative separation of immunoglobulins of the two allotypes. After fractionation of mixtures of anti-(T,G)-A--L antisera obtained from congenic strains differing in immunoglobulin allotype, it was possible to measure the antigen-binding capacity of specific anti-(T,G)-A--L antibodies in each allotype fraction. Analysis of artificial mixtures of immune sera obtained from homozygous Iga and Igb animals showed that this method is quantitative and internally consistent. This method of affinity chromatography was used in the analysis of specific anti (T,G)-A--L antisera from tetraparental mice.
Jones, P. P., Tacier-Eugster, H. and Herzenberg, L. A. (1974). “Lymphocyte commitment to Ig allotype and class.” Annales d Immunologie 125C(1-2): 271-6.
Herzenberg, L. A. and Jones, P. P. (1974). “Mouse immunoglobulin allotypes: characterization and use in cellular immunology.” Annales d Immunologie 125C(1-2): 71-83.
LAH #076-01
Herzenberg, L. A. and Okumura, K. (1975). “Mechanism of allotype suppression. pp. 93-104.” In: Singhal SK, Sinclair NR, ed. Suppressor cells in immunity. London, Univ of Western Ontario. http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?db=m&form=6&dopt=r&uid=0000784723
Herzenberg, L. A., Chan, E. L., Ravitch, M. M. and Riblet, R. J. (1973). “Active suppression of immunoglobulin allotype synthesis. 3. Identification of T cells as responsible for suppression by cells from spleen, thymus, lymph node, and bone marrow.” Journal of Experimental Medicine 137(6): 1311-24.
Goodman, J. R., Julius, M. H. and Herzenberg, L. A. (1973). Ultrastructural visualization of KLH antigen on selected mouse spleen lymphocytes. 2nd International Symposium on Electron Microscopy and Cytochemistry., Amsterdam, North-Holland Publishing,Co.
Julius, M. H., Sweet, R. G., Fathman, C. G. and Herzenberg, L. A. (1975). “Fluorescence-activated cell sorting and its applications. pp. 107-21.” In: Richmond CR et al., ed. Mammalian cells: probes and problems. Oak Ridge, Tenn., Technical Information Center. http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?db=m&form=6&dopt=r&uid=0000767737.
The Fluorescence Activated Cell Separator (FACS) separates cells according to fluorescence, light-scattering characteristics, or selected combinations of these two parameters. The instrument can process up to 5000 cells/sec and separate two nearly pure fractions with independently specified ranges of fluorescence and light- scattering cross sections for each fraction. The FACS can differentiate between viable and dead cells on the basis of light scattering. Appropriate threshold setting permits "gating out" of the dead-cell population so that cell populations can be separated or analyzed on the basis of viable-cell content. Using the FACS in combination with fluorescein-conjugated antigens to visualize antigen-binding cells enriched up to 400-fold from spleens of primed and unprimed mice. We have directly demonstrated that antigen-binding cells in both primed and unprimed mouse spleen contain the precursors of antibody-forming cells. Moreover, the antigen-binding precursor cells are functionally specific, and the avidity of the antibody formed is directly correlated with the avidity of the antigen receptors on the antigen-binding precursor cells.
Julius, M. H., Simpson, E. and Herzenberg, L. A. (1973). “A rapid method for the isolation of functional thymus-derived murine lymphocytes.” European Journal of Immunology 3(10): 645-9.
Kreth, H. W. and Herzenberg, L. A. (1974). “Fluorescence-activated cell sorting of human T and B lymphocytes. I. Direct evidence that lymphocytes with a high density of membrane-bound immunoglobulin are precursors of plasmacytes.” Cellular Immunology 12(3): 396-406.
Epstein, L. B., Kreth, H. W. and Herzenberg, L. A. (1974). “Fluorescence-activated cell sorting of human T and B lymphocytes. II. Identification of the cell type responsible for interferon production and cell proliferation in response to mitogens.” Cellular Immunology 12(3): 407-21.
Jones, P. P., Cebra, J. J. and Herzenberg, L. A. (1974). “Restriction of gene expression in B lymphocytes and their progeny. I. Commitment to immunoglobulin allotype.” Journal of Experimental Medicine 139(3): 581-99.
Bechtol, K. B., Wegmann, T. G., Freed, J. H., Grumet, F. C., Chesebro, B. W., Herzenberg, L. A. and McDevitt, H. O. (1974). “Genetic control of the immune response to (T,G)-A--L in C3H in equilibrium C57 tetraparental mice.” Cellular Immunology 13(2): 264-77.
Jones, P. P., Craig, S. W., Cebra, J. J. and Herzenberg, L. A. (1974). “Restriction of gene expression in B lymphocytes and their progeny. II. Commitment to immunoglobulin heavy chain isotype.” Journal of Experimental Medicine 140(2): 452-69.
Herzenberg, L. A. (1974). “Short-term and chronic allotype suppression in mice.” Contemporary Topics in Immunobiology 3: 41-75.
Cantor, H., Simpson, E., Sato, V. L., Fathman, C. G. and Herzenberg, L. A. (1975). “Characterization of subpopulations of T lymphocytes. I. Separation and functional studies of peripheral T-cells binding different amounts of fluorescent anti-Thy 1.2 (theta) antibody using a fluorescence-activated cell sorter (FACS).” Cellular Immunology 15(1): 180-96.
Julius, M. H. and Herzenberg, L. A. (1974). “Isolation of antigen-binding cells from unprimed mice: demonstration of antibody-forming cell precursor activity and correlation between precursor and secreted antibody avidities.” Journal of Experimental Medicine 140(4): 904-20.
Fathman, C. G., Small, M., Herzenberg, L. A. and Weissman, I. L. (1975). “Thymus cell maturation. II. Differentiation of three "mature" subclasses in vivo.” Cellular Immunology 15(1): 109-28.
Herzenberg, L. A., C.M. Metzler, L.A. Herzenberg (1974). Mechanism of allotype suppression in mice. Immunological Tolerance: Mechnaisms and Potential Therapeutic Applications. New York, Academic Press: 519-529.
Loken, M. R. and Herzenberg, L. A. (1975). “Analysis of cell populations with a fluorescence-activated cell sorter.” Annals of the New York Academy of Sciences 254: 163-171.
Herzenberg, L. A. (1974). “Allotype suppression and production(?) by thymocytes and thymus-derived cells. pp. 111-8.” In: Brent L, Holborow J, ed. Progress in immunology II. Vol 2: Biological aspects I. Amsterdam, North-Holland. http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?db=m&form=6&dopt=r&uid=0000763458.
Herzenberg, L. A. (1974). Chairman's Introduction, Symposium G 2nd International Congress on Immunology, Brighton, England, July 22-26, 1994. Progress in Immunology. Amsterdam, North holland American Elseveir. 2: 109.
Bechtol, K. B., Freed, J. H., Herzenberg, L. A. and McDevitt, H. O. (1974). “Genetic control of the antibody response to poly-L(Tyr,Glu)-poly-D,L-Ala--poly-L-Lys in C3H--CWB tetraparental mice.” Journal of Experimental Medicine 140(6): 1660-75.
Herzenberg, L. A., Okumura, K. and Metzler, C. M. (1975). “Regulation of immunoglobulin and antibody production by allotype suppressor T cells in mice.” Transplantation Reviews 27: 57-83.
Weissman, I. L., Small, M., Fathman, C. G. and Herzenberg, L. A. (1975). “Differentiation of thymus cells-1,2.” Federation Proceedings 34(2): 141-4.
Herzenberg, L. A. and Sweet, R. G. (1976). “Fluorescence-activated cell sorting.” Scientific American 234(3): 108-17.
Stout, R. D., Yutoku, M., Grossberg, A., Pressman, D. and Herzenberg, L. A. (1975). “A surface membrane determinant shared by subpopulations of thymocytes and B lymphocytes.” Journal of Immunology 115(2): 508-12.
Utilizing a quantitative fluorescence assay with the fluorescence-activated cell sorter (FACS), we have demonstrated that a rabbit antiserum obtained by immunization with cells of a mouse IgM-producing plasma cell tumor (MOPC104E) is reactive with at least two surface determinants, designated Th-B and ML2, on subpopulations of normal murine lymphocytes. The ML2 determinant is restricted to B lymphocytes. The Th-B determinant is shared by splenic B lymphocytes and a large subpopulation of thymocytes, the latter of which express a 3-fold higher density of Th-B on their surface than do the B lymphocytes. Neither Th-B nor ML2 were found on peripheral T cells or on brain, liver, or kidney cells. The available evidence suggesting that Th-B may be a stem cell determinant that is lost upon maturation is discussed.
Herzenberg, L. A., C.M. Metzler (1974). Antibody induced suppressor T-cells. The Immune System, Genes, Receptors, Signals. W. Sercarz, Fox. New York, Academis Press: 455-469.
Okumura, K., Metzler, C. M., Tsu, T. T. and Herzenberg, L. A. (1976). “Two stages of B-cell memory development with different T-cell requirements.” Journal of Experimental Medicine 144(2): 345-57.
We present evidence here for two stages in B-memory cell development, the first of which is T independent and the second T dependent. For these studies, we use a new type of T-deficient mouse (allotype suppressed) which specifically lacks T-helper activity (Th) for a subset of memory B cells responsible for approximately 10% of the overall IgG antibody response. We have shown elsewhere that these mice (SJL X BALB/c hybrids suppressed for Ig-1b) lack Th capable of helping Ig-1b memory cells, although they have normal Th activity for all other IgG memory B cells. This selective Th deficiency allows study of the effects of T depletion on memory development and avidity maturation of one population of B cells under conditions where the bulk of the immune response in the animal is proceeding normally, thus obviating environmental problems due to secondary effects of T depletion. With this sytem, we show that after a single priming dose of 2,4-dinitrophenyl-keyhole limpet hemocyanin, the memory B-cell pool in suppressed and nonsuppressed donors is indistinguishable with respect to magnitude and avidity of the response for all IgG antibodies produced, including Ig-1b antibody, despite the fact that expression of Ig-1b memory cells is prevented in intact Ig-1b-suppressed mice by the absence of Th capbale of cooperating with these memory cells. We have shown elsewhere that virtually all of the Ig-1b memory is carried by Ig-1b bearing cells. In contrast with the lack of suppressor T-cell effect on initial Ig-1b memory cell development, our data show that continued Ig-1b memory development is selectively impaired in suppressed mice. When primed mice are boosted repeatedly with the priming antigen, the average avidity of most of the IgG memory cells increases over 100-fold while there is no avidity increase in the Ig-1b component. To explain these data, we suggest that the development of high avidity memory occurs in two stages. The first stage, which occurs as a result of primary antigenic exposure, is the creation of a pool of IgG-bearing memory cells with a relatively low average avidity for the antigen. The appearance of these first stage memory cells does not require help from (post-thymic) Th, although Th are required for the expression of these memory cells (antibody production). The second stage of B-memory development requires both further antigenic stimulation and B-memory cell interaction with competent Th. This is a continuing process in which the number of memory cells in the pool remains relatively constant but the average avidity of these cells increases with continued antigenic exposure.