Herzenberg Procedures Binder (PDF)

Lab Procedures (PDF)

            Freezer Cleaning Procedures

            Hood Cleaning Procedure

            Mouse IV Injection Procedure

Lab Templates (Word)

            Mouse Bleeding

            Mouse Roster

            Mouse Treatment

            Weekly Stock Production (Stock-Exp. Rm)

            Weekly Stock Production (Breeding Room)

            Daily Table

FACS Templates (Word)

            Laser Cooling System

            ARIA

            FACSCAN

            FACSTAR

            FLASHER II

            LSRSCAN

            VANFORD

            VANTOO

FACS Staining Protocols

            Intracellular Cytokine FACS Staining

            Human Antibody Titration Protocol

Titration Analysis Worksheet (Word Document)

                        Titration Analysis Protocol (Word Document)

                        Iwan's Interactive Titration Tutorial

            MCB Staining Protocol

            Standard FACS Surface Staining Protocol

            Mario's Conjugation Protocols

            Mario's Perspective on Compensation

Other Protocols

            Hemoglobin ELISA Protocol - Not finalized

            Preparation of PBMC from Buffy coats

            Collecting Mouse Tissue Samples (Spleen and Perc)

            MACS Depletion Protocol -

            Freezing and Thawing PBMC

            Tietze Analysis Protocol

Lab Procedures

            Using the Conductivity Meter

            Using the Coulter Counter

Useful Documents

         Clinical Samples Log

Human Blood Guidelines

Human Blood Sample Record (Word Document)

         Human Blood Sample Labels (Word Document)

         11-color FACS experiment template (Word Document)

          Converting Filemaker to Excel (Word Document)

Recipes

          Bimane Media

Preparation of TC and FACS Staining media

ELISA Substrate Buffer

Avertin Anesthesia

Media

Lab Assistant Protocols

Miscellaneous