Herzenberg Procedures Binder

Lab Procedures

Freezer Cleaning Procedures

Hood Cleaning Procedure

Mouse IV Injection Procedure

Lab Templates

Mouse Bleeding

Mouse Roster

Mouse Treatment

Weekly Stock Production (Stock-Exp. Rm)

Weekly Stock Production (Breeding Room)

Daily Table

FACS Templates

Laser Cooling System

ARIA

FACSCAN

FACSTAR

FLASHER II

 LSRSCAN

VANFORD

VANTOO

FACS Staining Protocols

Intracellular Cytokine FACS Staining

Human Antibody Titration Protocol

Titration Analysis Worksheet

Titration Analysis Protocol

Iwan's Interactive Titration Tutorial

MCB Staining Protocol

Standard FACS Surface Staining Protocol

Mario's Conjugation Protocols

Mario's Perspective on Compensation

Other Protocols

 Hemoglobin ELISA Protocol - Not finalized

Preparation of PBMC from Buffy coats

Collecting Mouse Tissue Samples (Spleen and Perc)

MACS Depletion Protocol -

Freezing and Thawing PBMC

Tietze Analysis Protocol

Lab Procedures

Using the Conductivity Meter

Using the Coulter Counter

Useful Documents

Clinical Samples Log

Human Blood Guidelines

Human Blood Sample Record (Word Document)

Human Blood Sample Labels (Word Document)

11-color FACS experiment template (Word Document)

Converting Filemaker to Excel (Word Document)

Recipes

Bimane Media

Preparation of TC and FACS Staining media

ELISA Substrate Buffer

Avertin Anesthesia

Media