Herzenberg Procedures (From Binder) |
B Cell Blind Panel Transfectant Results
Cell Treatment With Glass Wool
Counting Cells With an Electronic Counter
Separation of Lymphocytes Using Ficoll-Hypaque
Method for Velocity Sedimentation Ig Gradient
Ficoll-Isopaque Technique (article)
Identification of Dead Cells in FACS Cloning Procedure
ulbecco Phosphate-Bufferred Saline (PBS)
Ethylenediamine Tetraacetic Acid (EDTA or Versene) pH 7.2
Acid Citrate Dextrose Solution
Procedure for IsoMEM Preparation
Antibiotics for Irradiated Mice
Preparation of Glutamine for Tissue Culture Media
Preparation of Concentrated Pen-Strep for Frozen Aliquots
To Calculate Molarity of a Solution When Only Limited Information is Given on Label
Procedure for Cell Separation by Nylon Wool Columns
Dupont Procedure for Wool Columns
Preparations for Cell Separators
TNP Conjugation to BRBC for Hemaglutination and Plaquing (updated for BRBC)
TNP Conjugation to SRBC for Hemaglutination and Plaquing
CoCl3 Coupling of HGG, KLH and POL to SRBC (Conjugate with CRCL3 on Day of Use)
Preparation of KLH, HGG Coated Gluteraldehyde Fixed SRBC for Hemagglutination
Use of Biotin-Activated Esters for Protein Conjugation
Preparation of Human Leukocyte Cultures for Chromosome Analysis
Preparation of Tissue Culture cells -H3 Thymidine-Pulsed Prior to Counting in Scintillation Counter
Ultrogel A - Ultrogel AcA Instruction Sheet
Absorption of Antibody with Lymphoid Cells
Quantitation of Immunoglobulins by Inhibition of Precipitation of I-125 Labelled Antigens
Cytotoxicity Assay - Cr51 Release
Agar Plates for Phage Inhibition Assay
Procedure for Phage Inhibition
Media for Phage Inhibition Assay
Phosphate-Gelatin Buffer (0.05M)
Precoating and Pouring Agar Slides
Procedure for Mouse Ouchterlony
Mishell-Dutton Culture Methods
Membrane Fluorescent Antibody Staining
Cytoplasmic Fluorescent Antibody Staining
Stripping of Surface Globulin in Mouse Cells
Pronase Stripping of Membrane Immunoglobulin in Rabbits
Wright Giemsa Stain (Blood Smears)
Autoradiography of Cell Smears
Chemical Identification of Monocytes
Cytochemical Identification of Monocytes and Granulocytes (article)
Fixation of Cells with Glutaraldehyde
Immunoprecipitation of Radiolabelled Proteins from NP-40 Extracts
S35-Methionine Labelling of Lymphocytes and NP-40 Extraction of Membrane Proteins
TCA Precipitation for Determinaion of Radioactivity
Solid Phase I-125 Labelling Protocol Page
Coupling Protein to Microspheres
Binding of Microspheres to Hybrind Cells
Standard protein-Microsphere Coupling Procedure
Sepracell-MN Separation Manual
Preparation of Brushite and Hydroxylapatite
Mycoplasma Screening of Cell Lines Using H33258 Staining of a 3T3 Indicator Cell Layer
Two-Dimensional Gel Electrophoresis
Operation of Packard Liquid Scintillation Counter
X-Irradiation Procedure for Mice
Preparation of Polaroid 3x5 Lantern Slides
Operation of Cavalli's Cell Freezing Apparatus
(Microscope) Photographing Settings For Slides
Changing UV Light on Wild Scope (Left Hand Scope)
Changing Bulb on Right Hand Scope
Washing Dialysis Membrane from Marbrook Cultures
Physiochemical data of _____ used in the adoptive transfer of the secondary _____ immune response
Myeloma Proteins = Groups = Allotypes (Class)
International Centrifuges - Nomograph for Computing Relative Centrifugal Force
Instructions for H-P Calculation of I125 Assay
Mouse Skin Grafting Procedure (Aplit Thickness Method)
Skin Grafting (New Procedure - 1981)
Stock Room - Mating and Weaning
Colormetric (MTT) Assay for Cell Survival and Proliferation
Autoradiography of Cell Smears
Preparation of Fluorescein or Rhodamine Labelled Chicken Red Blood Cells (CRBC)